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. 2023 May 30;19(5):e1011383. doi: 10.1371/journal.ppat.1011383

Fig 8. Overexpressed IFITM1 causes cholesterol accumulation at AiV RNA replication sites.

Fig 8

(A–E) Vero-IFITM1 cells were incubated with or without Tet for 48 h and then treated with mock (A and D), 1 μM 25-HC (B), 1.5 μM U18666A (C), or 150 nM BF738735 (E), followed by pCMV-polyprotein transfection. At 24 h after transfection, the cells were fixed and stained with filipin III (A–C) or anti-PI4P (D and E), anti-IFITM1 and anti-2B antibodies. Bars, 4 μm. Pearson’s correlation coefficient was measured for individual cells.