Table 2.

Optimized experimental parameters for ADC analyses by iCE280.

Ampholyte pH range	Pharmalytes 8–10.5/pharmalytes 3–10
Sample concentration	0.3, 0.5, 0.8, 1, and 1.5 mg/ml
Urea concentration	0, 1, 2, and 3 M
Focusing time	7, 10, 12, and 15 min
% ampholytes	4%, 6%, and 8%