Fig. 5. Everolimus-induced Myc-dependent cell death and immune infiltrations in the polyps.

APC^Min/+ or APC^Min/+/4ES209A (KI) mice with established polyps were treated with Everolimus for 3 days. The intestinal polyps were analyzed. A Representative Myc, TUNEL, p-βcat, p-eIF2α IF staining. Bar = 100 µm. B Quantitation of markers per 400× field from A. Nuclear Myc was scored. The counts in polyps from APC^Min/+ mice were included as controls for those in KI mice. C qRT-PCR analysis of the indicated genes. The relative levels were normalized control polyps in APC^Min/+ mice. D Representative Ly-6B.2 and CD68 IF staining. E Quantitation of cells from (D). B, C, E, n = 3 mice. ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001, ⁺⁺⁺⁺P < 0.0001 (one-way ANOVA and Tukey Post-Hoc test).