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. 2023 Jun 9;6:624. doi: 10.1038/s42003-023-05003-w

Fig. 2. Inhibition of mPFC-BLA circuit mimicked exercise effect of anxiolysis.

Fig. 2

a Time schedule of chemogenetic manipulation. b Upper, experimental scheme of viral injection. Lower, labeling of BLA-projecting neurons in prelimbic region (PrL). Scale bar, 100 μm. c Representative traces of mPFC neurons under 150 pA depolarizing currents. d Number of spikes under gradient depolarizing currents. Two-way ANOVA with respect to the group effect, F(3,400) = 21.04, P < 0.0001. n = 11 neurons from 4 mice in each group. e Total distance in the open field. One-way ANOVA, F(3,28) = 1.852, P = 0.1067. f Central duration in the open field. F(3,28) = 8.140, P = 0.0005. g Total distance in the elevated plus-maze. F(3,28) = 0.4935, P = 0.6897. h Time duration in the open arm. F(3,28) = 17.82 P < 0.0001. N = 8 mice in each group in (e–h). ns, no significant difference; **P < 0.01, ****P < 0.0001. All data were presented as mean ± sem.