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. 2023 Jun 9;6:624. doi: 10.1038/s42003-023-05003-w

Fig. 3. Reactivation of mPFC-BLA pathway blocked exercise anxiolytic effect.

Fig. 3

a Time schedule of chemogenetic manipulation. b Upper, experimental scheme of viral injection. Lower, labeling of BLA-projecting neurons in PrL. Scale bar, 100 μm. c Representative traces of PrL neurons under 150 pA injected currents. d Number of spikes under gradient depolarizing currents. Two-way ANOVA with respect to the group effect, F(4,520) = 34.39, P < 0.0001. n = 11 neurons from 4 mice in each group. e Total distance in the open field. One-way ANOVA, F(4, 35) = 0,2056, P = 0.9336. f Central duration in the open field. F(4,35) = 9.986, P < 0.0008. g Total distance in the elevated plus-maze. F(4,35) = 0.05699, P = 0.9937. h Time duration in the open arm. F(4,35) = 15.03, P < 0.0001. N = 8 mice in each group in (e–h). ns, no significant difference; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. All data were presented as mean ± sem.