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. 2023 Jun 9;14:3240. doi: 10.1038/s41467-023-38704-1

Fig. 2. Impact of village culturing system.

Fig. 2

a Experimental design to test the impact of village culturing systems on hiPSC transcriptional profiles. The same experiment was carried out at each of the three different sites in triplicate. b The proportions of each of the three hiPSC lines at each of the three different sites from uni- and village cultures. Error bars show the standard error of the triplicates around the mean. c Histograms of the variance of gene expression that is explained by the covariates and the interaction of the covariates measured. The lines below the histogram each represent a gene for that covariate. d The variance of important stem cell markers explained by the covariates. e Important stem cell marker expression for each hiPSC line at each Site. Differential expression was calculated with logistic regression and significance was corrected for multiple comparisons. hiPSC: human induced pluripotent stem cell; *adjusted P-value < 0.05 for differential expression.