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. 2023 Jun 9;14:3240. doi: 10.1038/s41467-023-38704-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Experimental design for testing a large village of 18 unrelated hiPSC lines including the cell numbers and transcriptional profiles during cardiomyocyte differentiation and hiPSC village maintenance. b Proportion of cells from each hiPSC line during cardiomyocyte differentiation (15-day protocol). c Proportion of cells from each hiPSC line during hiPSC village culture maintenance. d Proportion of gene expression variance during hiPSC village maintenance explained by the covariates measured in this dataset—Line and Passage. Each line in the rug plot below the histogram shows the observation of a gene for that covariate. e Replication of eQTLs previously described by DeBoever et al. with the 18-line iPSC village with 83% of eQTLs in concordant directions. Significance identified with two-sided Chi-squared test.