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. 2023 Jun 9;14:3395. doi: 10.1038/s41467-023-39082-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Time-series confocal imaging of human pancreas in A-ha copolymer (a–c, solid state), Ce3D clearing liquid (d–f, aqueous solution), and CytoVista clearing liquid (g–i, organic solvent). a–c, d–f, g–i are representative images and quantitative analysis of photobleaching. Tissues were labeled with DAPI (white, control), anti-S100B (green, standard immunohistochemistry; control), and anti-CD31 (red, AF-647-conjugated primary antibody; indicator) for the antifade test. ×40 objective was used to acquire 500 frames from a 320 × 320-µm region (~30 µm under tissue surface, six repeats per data point). In c, f, i, data are expressed as % of fluorescence intensity at cycle = 1 (means with standard deviation). ***p < 0.001; **p < 0.01; n.s., nonsignificant vs. A-ha at cycle = 500 (two-sided unpaired Student’s t test). Source data are provided as a Source Data file.