Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 9;14:3410. doi: 10.1038/s41467-023-39156-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a The viability of 57 human cell lines were evaluated by an MTT assay 72 h after infection. Liver cancer is shown in yellow, colon cancer in green, prostate cancer in orange, pancreas cancer in purple, bladder cancer in blue and breast cancer in light blue. b The oncolytic effects of M1-GFP and M1-N3E2M were analyzed by Two-tailed paired t test and P values are indicated. The data are shown as violin plots with the box limits at minima and maxima and center line at median. (colon cancer, n = 8; liver cancer, n = 2; breast cancer, n = 15; pancreas cancer, n = 11; prostate cancer, n = 6; bladder cancer, n = 15). c, e HCT-8 and Huh-7 cells were infected with M1 viruses at an MOI of 0.1. Representative images of n = 3. Scale bars, 50 μm. d, f The viability of HCT-8 and Huh-7 cells was evaluated by an MTT assay. EC50 shift was calculated by nonlinear regression. Statistical significance was calculated using Two-way ANOVA with Sidak’s multiple comparisons test relative to M1-GFP. Adjusted P values are: d MOI (Mock), P > 0.9999; MOI (−3), P = 0.9991; MOI (−2), P = 0.0008; MOI (−1 to 1), P < 0.0001; f MOI (Mock), P > 0.9999; MOI (−3), P = 0.0528; MOI (−2), P = 0.0028; MOI (−1 and 0), P < 0.0001; MOI (1), P = 0.0489. g CCD-18Co cells were infected with M1-GFP and M1-N3E2M at an MOI of 10. Representative images of n = 3. Scale bars, 50 μm. h The viability of CCD-18Co cells was evaluated by an MTT assay. EC50 shift was calculated by nonlinear regression. n.s.: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data points represent mean % viability relative to vehicle ± SD, for n = 3 biological replicates. Source data are provided as a Source Data file.