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. 2023 Jun 9;14:3407. doi: 10.1038/s41467-023-38824-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic of the normothermic perfusion system for diagnostic assessment of fresh untreated (control), 24-h UW preserved (24 h), VMP loaded-unloaded (VMP), VS55 loaded-unloaded (VS55), and vitrified and nanowarmed (V–N) rat kidneys. b Cumulative urine output over the course of perfusion. c Creatinine clearance. d Alterations in renal artery flow. e Oxygen consumption rate. f Venous pH. g Vascular resistance. h Lactate production. i Glucose consumption. Data are mean ± s.d. n = 4 per condition. Statistical comparison between nanowarmed kidney and other treatment groups at minute 40 using ANOVA and Tukey HSD (Games–Howell if unequal variance) post hoc tests. *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant. Full statistical treatment in Supplementary materials. Source data are provided as a Source Data file. OCR oxygen consumption rate; V–N, vitrified and nanowarmed.