Fig. 4. In vivo elimination of subcutaneous HCC tumors by HSV-1 and Navoximod loaded silk-hydrogels (V-Navo@gel).

a Scheme of the therapeutic procedure. b, c Tumor volumes of mice with indicated treatments. The number in the brackets indicated the tumor objective response rate (ORR) of the mice in each group after the whole monitoring process (n = 6 mice per group; Data were shown as means ± SEM). d Tumor images isolated from mice after the indicated treatments. e The survival curves of mice with indicated treatments (n = 6; Data were shown as means ± SEM). f Inoculated mice weight changes during the whole measurement (n = 6; Data were shown as means ± SEM). g Scheme of the tumor rechallenge model. h, i Tumor volumes of mice with indicated treatments (n = 8; Data were shown as means ± SEM). j Tumor images isolated from mice after the indicated treatments. k Representative FACS plots (gated on CD4⁺ or CD8⁺cells) and the percentage of T_em (CD62L^lowCD44^high), T_cm (CD62L^highCD44^high) and naïve T cells (CD62L^highCD44^low) were examined by FACS (n = 3; Data were shown as means ± SEM).