Figure 2.

CircSH3PXD2A inhibits chemoresistance of SCLC. (A) The expression levels of circSH3PXD2A and SH3PXD2A were detected after transfection with circSH3PXD2A lentivirus or siRNA in SCLC cells. (B) Chemoresistance assays were performed to analyze the chemoresistance of SCLC cells to the chemotherapeutic drugs DDP, ADM, and VP-16. (C) Flow cytometric analyses for apoptosis in SCLC cells transfected with lentivirus or siRNA after DDP treatment. (D) CCK-8 assay was employed to analyze the effect of circSH3PXD2A on SCLC cell proliferation. (E) After transfected with circSH3PXD2A siRNA or negative control, the migration and invasion capacities of H446 cells were detected through transwell assays. Scale bar, 100 μm. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Abbreviations: ns, no significance; OE, overexpression; si, siRNA.