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. 2023 Jun 5;18:2989–3006. doi: 10.2147/IJN.S407116

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© 2023 Chao et al.

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Direct regulation of SCLC chemoresistance through circSH3PXD2A/miR-375-3p/YAP1 axis in vitro. (A) The mRNA expression levels of YAP1 in SCLC cells transfected with miR-375-3p mimic (left) or inhibitor (right). (B) Western blotting was conducted to analyze the effect of miR-375-3p on the expression level of YAP1. (C and D) Pull-down assay revealed the enrichment of YAP1 by biotin-labeled miR-375-3p mimic. (E) Schematic diagram showing the site at which miR-375-3p binds to the 3’UTR of YAP1, and a luciferase reporter assay suggested the interaction of YAP1 with miR-375-3p. (F and G) Determination of the mRNA (F) and protein (G) expression levels of YAP1. (H) The protein expression level of SCLC cells lentivirally transfected with vector, circSH3PXD2A or circSH3PXD2A combined with CA3 (1 μM) was detected with Western blotting. (I and J) Effects of YAP1 on the proliferation (I) and chemoresistance (J) of SCLC cells. (K) Comparison of YAP1 levels in SCLC patients and healthy people. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Abbreviation: ns, no significance.