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. 2023 Jan 30;24(7):725–741. doi: 10.1111/mpp.13299

FIGURE 2.

FIGURE 2

ZmLOX10 functions to suppress salicyclic acid (SA) and enhance jasmonic acid (JA) accumulation resulting in reduced resistance of opr2‐1 to Colletotrichum graminicola. (a) Disease symptoms of wild type (WT), opr2‐1, lox10‐3, and lox10opr2 7 days postinoculation (dpi) with C. graminicola. Disease symptoms were scanned and lesion areas were measured using ImageJ software. The data are shown in the box and whisker plot and X indicates means (n = 36 lesions from six different plants of each genotype as biological replicates). Outliers are represented by dots. This experiment was repeated at least three times with similar results. Contents of (b) SA, (c) 12‐OPDA, (d) JA, and (e) JA‐Ile were measured at 0, 1, 3, and 5 dpi of the WT, opr2‐1, lox10‐3, and lox10opr2 mutants. JA and JA‐Ile were at undetectable levels at 0 and 1 dpi. Bars are mean ± SEM (n = 5 maize plants of each genotype as biological replicates). (f) opr2‐1, lox10‐3, and lox10opr2 mutants and their WT were sprayed with 2 ml of mock or 100 μM MeJA per plant 1 h prior to inoculation with C. graminicola. The data are shown in the box and whisker plot and X indicates means (n = 36 lesions from six different plants of each genotype as biological replicates). Different letters indicate statistically significant differences among the genotypes (Tukey's HSD test, p < 0.05). Metabolite measurement was repeated at least two times with similar results.