Figure 3.

Relationship between viscosity contrast and electrodeformability of GUVs. Different PEG 8000 concentrations were encapsulated inside GUVs to vary viscosity contrast between GUV lumen and outer solution. (A) Bright-field images showing PEG 8000 GUV shape transitions from undeformed to elliptically deformed to spherical recovery. (Top) Electroperturbation of 2% w/v PEG 8000 GUV. (A1) A PEG 8000 GUV at an undeformed state prior to AC field application. (A2) Steady-state deformation of GUVs during electoperturbation. (A3) GUV after electrodeformation recovery to assume spherical shape. (B1B3) 4% w/v PEG 8000 GUV electrodeformation. (Bottom) Electroperturbation of 8% w/v PEG 8000 GUV. (B) Deformation profile of 2% and 4% w/v PEG 800 GUVs, n = 3. Shaded rectangular box denotes approximate duration of electric field application. Shaded areas in the traces in indicate ±SD. (C) Comparison and statistical analysis of maximum GUV deformation of each GUV conditions indicated. Note that max a/b ratio of 8% was 1 for all vesicles analyzed and thus has no error bars. Data represent mean maximum deformation, and error bars denote ±SE. n_2% = 13, n_4% = 11, and n_8% = 12. Scale bars, 10 μm. To see this figure in color, go online.