Table 1.
Description of all Primers Used in this Research
| For Mutagenesis | |
|---|---|
| K364 forward | aggggttatgacgaaacaaa |
| K364M reverse | cccgcccatacagtgaatcgc |
| K364I reverse | cccgcctatacagtgaatcgc |
| K364A reverse | cccgcctgcacagtgaatcgc |
| K364Q reverse | cccgccttgacagtgaatcgc |
| K364R reverse | cccgcctctacagtgaatcgc |
| C363S reverse | gcctttgctgtgaatcgctatcac |
| K364L forward | cactgtttaggcgggaagggaaga |
| K364L reverse | cccgcctaaacagtgaatcgctat |
| G214C forward | gccgattgtttggggagattggtt |
| G214C reverse | ccccaaacaatcggctcctgtttc |
| K303M forward | cactgcatgggaggaaagggtcgg |
| K303M reverse | cctcccatgcagtggatggcaata |
| Ci-Md-VSP forward | ctcgagatggagggattcgacggttc |
| Ci-Md-VSP reverse | ttaaaacagtagttccactgcgccggcg |
| Ci-Md-VSP joint forward | gtccaggtcaaatccatctttcctgtatcgacgctt |
| Ci-Md-VSP joint reverse | aagcgtcgatacaggaaagatggatttgacctggac |
| Ci-Md-VSP∗ forward | gaatgatatcagaaaacaagcgtcgatacaggaa |
| Ci-Md-VSP∗ reverse | tttctgatatcattcttctgcttgaagccttcatttg |
| For Opossum cDNA Isolation | |
|---|---|
| UTR5 Md-VSP forward | aagaaaagatgaaaaaacctc |
| UTR3 Md-VSP reverse | cccattcaataaacatttaat |
| Md-VSP NotI forward | ttttgcggccgcatggaagaaaaagagcacgg |
| Md-VSP Acc65I reverse | tttggtaccttaaaacagtagttccactg |