Table 1.

List of parameters used in this work

Variables	Parameters details
Confocal volume calibration parameters
$w_{xy}$	width of the observation area in x, y direction
$s=w_z/w_{xy}$	shape factor of the 3D Gaussian excitation point spread function
Parameters used in ACF fit
$G\left(\tau \right)$	auto-correlation function (ACF)
${\tau }_{1/2}$	characteristic time of half temporal decorrelation; i.e., $G\left({\tau }_{1/2}\right)=G\left(\infty \right)+\left(G\left(0\right)-G\left(\infty \right)\right)/2$
${\tau }_d$	characteristic diffusion time of particles in the confocal volume, ${\tau }_d={\tau }_{1/2}$ for 2D diffusion
$N$	effective number of particles in confocal volume
$T$	fraction of particles in triplet state
${\tau }_T$	characteristic residence time in triplet state
Parameters of the 2D/3D diffusion and binding kinetics
$D_{2\mathrm{D}}$	diffusion coefficient of membrane-bound molecules
$D_{3\mathrm{D}}$	diffusion coefficient of free molecules
$k_{\text{on}}$	association constant of particles to the membrane
$k_{\text{off}}$	dissociation constant of particles from the membrane
$K_D=k_{\text{off}}/k_{\text{on}}$	binding affinity of the proteins to the membrane
$K_P={\left[F\right]}_{\mathrm{e}\mathrm{q}}/{\left[B\right]}_{\mathrm{e}\mathrm{q}}$	Experimental apparent partition coefficient (with ${\left[F\right]}_{\mathrm{e}\mathrm{q}}$ and ${\left[B\right]}_{\mathrm{e}\mathrm{q}}$ the equilibrium concentrations of free and bound proteins, respectively).