Fig. 2.
Benchmarking of the host cell protein (HCP) Profiler standard against the Mix 4P using a MS1-XIC data-dependent acquisition (DDA) approach. Comparison of global HCPs numbers and amounts obtained for (A) post protein A affinity chromatography (PPA) and (B) harvest fractions on a Q-Exactive HF-X using both methods. Bar heights represent the means of the global HCP amounts in injection triplicates. Error bars represent the standard deviation. Dots indicate the numbers of quantified HCPs.