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. 2023 Mar 31;13(5):494–502. doi: 10.1016/j.jpha.2023.03.009

Fig. 2.

Fig. 2

Benchmarking of the host cell protein (HCP) Profiler standard against the Mix 4P using a MS1-XIC data-dependent acquisition (DDA) approach. Comparison of global HCPs numbers and amounts obtained for (A) post protein A affinity chromatography (PPA) and (B) harvest fractions on a Q-Exactive HF-X using both methods. Bar heights represent the means of the global HCP amounts in injection triplicates. Error bars represent the standard deviation. Dots indicate the numbers of quantified HCPs.