TABLE 4.
Top 10 taxa at a phylum level across all female samples (n = 4 for each sample) in the gut ecosystem of D. rerio.
| ASV | RF F1 | RF F2 | RF F3 | RF F4 | SR F1 | SR F2 | SR F3 | SR F4 | RP F1 | RP F2 | RP F3 | RP F4 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Proteobacteria | 90.2 | 96.6 | 93.9 | 96.6 | 88.9 | 76.8 | 91.6 | 92.6 | 69.2 | 97.7 | 76.5 | 89.8 |
| Fusobacteriota | 0 | 0 | 0.3 | 0.5 | 0.4 | 1.7 | 0.1 | 0.1 | 0 | 0 | 0 | 0 |
| Firmicutes | 5.8 | 2.1 | 0.7 | 1 | 3.4 | 7.1 | 7.1 | 3.7 | 23 | 0.8 | 2.6 | 2.7 |
| Crenarchaeota | 0.8 | 0 | 0 | 0.1 | 0.8 | 2 | 0.2 | 0.1 | 0 | 0.1 | 1.2 | 0.5 |
| Actinobacteriota | 1.9 | 0.9 | 1.7 | 0.8 | 2.9 | 4.6 | 0.6 | 0.8 | 5.9 | 0.8 | 7.3 | 2.8 |
| Planctomycetota | 0.9 | 0.1 | 2.8 | 0.8 | 3.4 | 4.8 | 0.4 | 0.2 | 1.2 | 0.5 | 6.9 | 1.8 |
| Bacteroidota | 0.2 | 0.3 | 0.5 | 0.2 | 0.1 | 0.6 | 0 | 1 | 0.4 | 0 | 1 | 0.6 |
| Unassigned | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 2.5 | 1.5 |
| Verrucomicrobiota | 0.2 | 0 | 0.1 | 0 | 0.2 | 1 | 0 | 0.6 | 0.2 | 0.1 | 1.7 | 0.2 |
| Cyanobacteria | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0.1 | 0 | 0 | 0.2 | 0.1 |
Taxonomic identities were based on their assignment through the (SILVA v138) database as determined by the Quantitative Insights into Microbial Ecology (QIIME2, v2022.2). The mean of each sample group was displayed for clarity. Sample assignments are as follows: RF M = female D. rerio fed with the reduced-fat diet; SR F = female D. rerio fed with the standard reference diet; RP F = female D. rerio fed with the reduced-protein diet. A complete list of the output of QIIME2 taxa and their abundances is presented in Supplemental Data 1.
RF, reduced-fat diet; RP, reduced-protein diet; SR, standard reference diet.