Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Stability of mitoxantrone–DNA adducts at 37°C. (A) The 512 bp fragment was reacted with 25 µM mitoxantrone and 5 mM formaldehyde for 2 h at 37°C. Following ethanol precipitation, transcription was initiated prior to elongation for 5–150 min as shown at 37°C. (B) First order kinetic analysis of the loss of transcriptional blockage at individual adduct sites as indicated. The half-lives of adducts at individual sites are summarised in Table 1.
