Fig. 1. Drugs induce dose- and time-dependent changes in cell cycle behavior.

A Schematic of reporter with a bidirectional promoter driving expression of human DNA Helicase B (HDHB) fused to the green fluorescent protein clover, and a second transcript coding for NLS-RFP-NLS, a ribosome skipping domain (T2A), and a puromycin resistance protein. B Quantification of nuclear intensity of the cell cycle reporter in a cell and its progeny across time. The time of G₁-S transition and cell division are demarcated with black and red circles, respectively. C Schematic of the five drugs tested and the cell cycle phase they target. D Average growth curves of AU565 cells tracked every 30 min for 96H across an 8-point dose response for lapatinib, gemcitabine, paclitaxel, palbociclib, and doxorubicin. The null dose is colored red. Line traces show the average from three independent experiments. The black triangle indicates the addition of fresh drug and media. All doses in nM. E Percentage of cells in S-G₂ phase of the cell cycle across doses. 50 nM Lapatinib and 3 nM paclitaxel are colored blue. Color scheme as in 1D. F Representative GFP images at 39.5H for 250 nM lapatinib, 30 nM gemcitabine, 3 nM paclitaxel, 250 nM palbociclib, 20 nM doxorubicin from data plotted in D. Each experiment was performed in triplicate and achieved similar results. Source data are provided on Synapse.