Fig. 4. Responses to drug combinations are dependent on drug-specific effects on cell cycle and cell death.

A–D Comparison between model predictions (upper) and experimental observations (lower). Model predictions are based on standard Bliss additivity using cell numbers or through additive combination of single agent effects on cell rates. Experimental observations for the single drug responses and the drug combinations effect on cell numbers. A Drug responses for increasing doses of palbociclib and in combination with 100 nM lapatinib. B–D Drug responses for increasing doses of gemcitabine in combination with 20 nM doxorubin, 100 nM lapatinib, or 50 nM palbociclib. E Comparison of predicted and experimentally observed changes in cell cycle phase for single and combination treatments of lapatinib and gemcitabine. Upper panel shows model predictions and lower panel shows experimentally observed responses. Error bars show the standard error of the mean for three biological replicates. Source data are provided on Synapse.