Figure 2.

Primary data on the cleavage of fluorescein 5′-labelled S-WT by the wild-type hairpin ribozyme HP-WT (lane 1) and of fluorescein 5′-labelled S-WTSV1 by the hairpin ribozyme with varied sequence HP-WTSV1 (lane 2). Cleavage conditions: 20 nM ribozyme, 200 nM substrate, 10 mM Tris–HCl pH 7.5, 12 mM MgCl₂, 37°C. Lane 3 shows the migration of a separately synthesised fluorescein 5′-labelled RNA (5′-AGACA-3′) corresponding to the sequence of the 5mer fragment resulting from cleavage of S-WTSV1 by HP-WTSV1. cp, 2′,3′-cyclic phosphate. All lanes show autoscaled data (ordinate, fluorescence intensity in arbitrary units).