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. 2000 Feb 15;28(4):901–910. doi: 10.1093/nar/28.4.901

Figure 1.

Figure 1

Analysis of Rev-independent HIV-1 Gag expression. (A) Schematic diagram of plasmids pK-R-gpII, pK-gpII, pK-gpII-C and pK-gpII-CCCC. All plasmids contain part of the 5′-UTR and the coding region for the Gag and PR domains of HIV-1. Relevant RNA transport elements, either the RRE of HIV-1 or the CTE of M-PMV, are depicted as black boxes. All plasmids contain the SV40 large T antigen splice and poly(A) signal (pA). At the top, the position of the gag/PR region and of the RRE as well as the coding exons of Rev are highlighted in the HIV-1 genome. At the bottom, the relative position of the CTE is highlighted in the M-PMV genome. (B) Immunoblot analysis of transfected HeLa cells using antiserum against HIV-1 CA. Cells were transfected with plasmids pK-gpII (lane 1), pK-R-gpII (in the absence or presence of Rev; lanes 2 and 3), pK-gpII-C [CTE in the antisense (a.s.) or sense orientation; lanes 4 and 5] or pK-gpII-CCCC (lane 6). The Gag polyprotein (Pr55) and the CA protein are identified on the right; molecular mass standards (in kDa) are given on the left. The two additional proteins migrating between Pr55 and CA in lane 3 correspond to the Gag intermediate cleavage products MA-CA-NC and MA-CA.