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. 2023 May 29;14:1168292. doi: 10.3389/fimmu.2023.1168292

Figure 2.

Figure 2

Sudan black B (SBB) blocks autofluorescence of brain tissues damaged by intracerebral hemorrhage. (A) Untreated brain sections of the striatum after intracerebral hemorrhage. (B) The same brain sections were treated with 0.15% SBB and images were acquired showing the same area around the hematoma (A) before and (B) after SBB treatment. The images were visualized with three different microscopic filter sets. (C) The mean fluorescence intensity under the FITC, Tx Red, or DAPI filter in the same brain sections before and after SBB treatment. The percentage of mean fluorescence of the brain sections relative to the untreated brain sections is quantified in (C) Untreated vs. SBB treated: FITC, **p=0.0023; Tx Red, ****p<0.0001; DAPI, **p=0.0037; FInteraction=0.6899, FTime=2.081, FColumn Factor=51.23; two-way ANOVA; n=6 mice/group.