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. 2023 May 29;14:1168292. doi: 10.3389/fimmu.2023.1168292

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Copyright © 2023 Wang, Ren, Wang, Peng, Niu, Song, Li, Jiang, Zang, Zille, Fan, Chen and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sudan black B (SBB) blocks the autofluorescence of brain tissues damaged by traumatic brain injury. (A) Autofluorescence of brain tissues damaged by traumatic brain injury. (B) The same brain section was treated with 0.15% SBB. FITC, Tx Red, and DAPI filters were used to collect images from the same brain area in sections before and after SBB treatment. (C) Quantified mean fluorescence intensity of brain sections treated before and after SBB. The percentage represents the decrease in the mean fluorescence intensity after SBB treatment. Untreated vs. SBB-treated: FITC, *p=0.0227; Tx Red: *p=0.0316; DAPI: p=0.4786; F_Interaction=0.7227, F_{Row Factor}=6.746, F_{Column Factor}=15.92; two-way ANOVA; n=6 mice/group.