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. 2023 May 29;14:1168292. doi: 10.3389/fimmu.2023.1168292

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Copyright © 2023 Wang, Ren, Wang, Peng, Niu, Song, Li, Jiang, Zang, Zille, Fan, Chen and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sudan black B (SBB) blocks autofluorescence of brain tissues damaged by cerebral ischemia in a middle cerebral artery occlusion model. (A) Anti-NeuN antibody labeled with Alexa Fluor 488 was used to assess the immunofluorescence of brain tissues damaged by cerebral ischemia in the same brain sections before and after SBB treatment under a fluorescence microscope using the FITC filter. The white circles indicate representative regions of the significant difference in fluorescence signal before and after SBB treatment. (B) SBB treatment eliminated tissue autofluorescence by 64.18% compared to before treatment (*p=0.0253; t=3.154; paired t-test; n=6 mice/group).