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. 2023 May 29;14:1199040. doi: 10.3389/fimmu.2023.1199040

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Copyright © 2023 Ren, Zhang, Zhang, Feng, Li, Lin and Yu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The identification of CAF clusters according to scRNA data of ESCC patients. (A) tSNE plots of distribution of 32 clusters and fibroblasts-based marker genes expression. (B) tSNE plots of distributions of five fibroblasts after clustering. (C) Subgroups in cancer and adjacent tissue and proportion as well as cell number calculation. (D) KEGG analysis of five fibroblasts subgroups. (E) tSNE distribution of malignant and non-malignant cells predicted by copycat package. (F) Heatmap of the top5 marker gene expression of subgroups. (G) Bubble diagram of the top5 marker gene expression of subgroups. (H) Volcano plot of the top5 marker gene expression of subgroups.