Table 2.
Effect of Ceratonia siliqua concentrations 20, 30, and 40 μg/mL added to cryopreservation/thawing media on ROS intracellular, PMI, viability, MMP, and acrosome reaction of human spermatozoa
| Groups | Fresh-C | Frozen-C | C. siliqua 20 μg/mL | C. siliqua 30 μg/mL | C. siliqua 40 μg/mL |
|---|---|---|---|---|---|
| ROS intracellular | 40.1±6.3 | 59.1±6.1a) | 52.0±6.5b) | 52.3±6.1b) | 51.3±6.0b) |
| PMI | 53.1±3.5 | 24.4±3.9a) | 55.1±3.4b) | 41.3±3.9b) | 41.9±3.5b) |
| Viability | 71.3±1.5 | 45.3±1.09a) | 51.6±1.8b) | 50.5±1.3b) | 50.9±1.3b) |
| MMP | 65.5±4.2 | 33.6±4.7a) | 42.5±4.9b) | 44.2±4.9b) | 42.9±4.5b) |
| Acrosome reaction | 77.2±5.5 | 56.5±5.1a) | 61.5±5.9b) | 60.3±5.2b) | 60.9±5.7b) |
Values are presented as mean±standard error of the mean.
ROS, reactive oxygen species; PMI, plasma membrane integrity; MMP, mitochondrial membrane potential; C, control
a)
p<0.05 significant differences vs. fresh control group;
b)
p<0.05 significant differences vs. frozen control group.