Figure 5.

Enhancement of the Rpb1–Rpb3 interaction by Rpb8. Rpb1H and GST–Rpb3 were coexpressed with and without Rpb8. The expression was detected by western blotting with antisera against each subunit (cell lysate). The extracts of the coinfected cells were subjected to GSH-affinity chromatography and the eluted fractions were analyzed by western blotting (GSH affinity). The proteins coexpressed are indicated on the top: 1H, Rpb1H; G3, GST–Rpb3; 8, Rpb8, and the detected subunits are indicated on the left. Portions of the filters corresponding to the migration positions of each subunit in the SDS–PAGE are arrayed.