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. 2023 Mar 3;14(6):719–726. doi: 10.1021/acsmedchemlett.2c00479

Figure 4.

Figure 4

Additional M6Pn-conjugate uptake studies using the biotin/neutravidin system. A) Structure of conjugates 13 through 24. Conjugates 13 through 23 use the general tetrameric structure shown above the table, modifying both linker A and linker B for structure and composition. Conjugate 24 changes the multivalency to a hexameric structure. All conjugates contain a terminal biotin moiety. B) Fold change in MFI data from uptake studies of conjugates 17 through 24. Huh7 cells were incubated with 500 nM of neutravidin-650 (NA-650) and 10 uM of M6Pn-biotin at 37 °C for 24 h. The cells were then washed with PBS, and the uptake was determined by measuring the fluorescent intensity at 650 nm excitation/680 nm emission. Data is shown in relative fluorescent units.