Fig.4. inhibits growth of pancreatic tumor in orthotopic xenograft mouse models associated with downregulation of p53 and NCOA4 expression.

A, Top, Panc1luc cells were implanted orthotopically into the pancreas of SCID mice followed by MMRi62 treatment via intraperitoneal injection at doses of 25 mg/kg at every other day for 5 injections, 2 days/week for 2 weeks. The dorsal luciferase signals in the mice bearing Panc1luc orthotopic tumors were captured in different days during and after treatment. Middle, mouse body weight (left) and tumor BLI signal in Flux (Right) measured in indicated days in vehicle-control (V) mice and MMRi62-treated (M) mice were shown. Bottom, At the termination day of the experiments, median tumor weight in gram and median tumor size in cm in the two groups of mice were shown. *P =0.0002 and <0.00001 for Panc1 tumor weight and tumor size between V and M groups; and *P =0.001 and <0.0001 for BxPc3 tumor weight and tumor size between V and M groups. B, the same in A except for BxPc3luc cells. C, HE staining of Panc1luc and BxPc3luc orthotopic tumors collected on Day 37 in MMRi62-treated (M) as compared with vehicle-treated mice (V). D, WB analysis of the resected tumor tissues on Day 37 for the expression of p53 and NCOA4 in V and M group of mice. E, Immunofluorescence staining of activate caspase 3 (AC3) for in Panc1luc and BxPc3luc orthotopic tumors collected on Day 37 in MMRi62-treated (M) as compared with vehicle-treated mice (V).