Fig. 2.

The density of cholinergic axons is significantly reduced in the cortex of App^NLGF mice. (A) Representative images of the cortex from frozen coronal brain sections immunostained with antibodies against VAChT (green). FSB was used for detecting Aβ plaques (magenta). Scale bars, 100μm. (B) VAChT immunoreactivity was quantified and expressed as positive area (%). n = 4 /group. ^***p < 0.001. (C) A higher magnification of framed region indicated in A. Scale bar, 20μm. (D) Representative images of the NBM from frozen coronal brain sections immunostained with antibodies against ChAT (green) were shown (magenta indicated PI staining). FSB was used for detecting Aβ plaques (blue). (E) The number of ChAT-positive cells was counted and expressed as cell number per area (/mm²). Scale bar, 100μm. n = 4 /group. ns, not significant.