Fig. 5.

p-tau181-positive axons do not overlap with glutamatergic axons in mouse brains. Representative images of the cortex (A1– C3) and hippocampal CA1 subfield (D1– F3) from frozen coronal brain sections immunostained with antibodies against VGLUT1 (magenta) and p-tau181 (green). FSB was used for detecting Aβ plaques (blue). Scale bars, 100μm. (A4– C4) Higher magnification of framed regions indicated in A3– C3. Scale bars, 20μm. (A5 and C5) Higher magnification of framed regions indicated in A4 and C4. Scale bars, 2.5μm. (G) VGLUT1 immunoreactivity in the cortex or hippocampal CA1 subfield was quantified and expressed as relative percentage to WT. n = 4 /group. ns, not significant.