Fig. 1.

Detection of PE-mediated nucleotide substitutions in sheep. A Schematic view of the generation of prime-edited lambs via the PE3 system. pegRNA: prime editing guide RNA and sgRNA: single guide RNA. B Schematic view of the target site in the sheep BMPR1B and TBXT genes. PBS: primer binding site, RT: reverse transcriptase template, and PAM: protospacer adjacent motif. C Targeted editing efficiency in HEK293T cells and newborn lambs. WT: wild-type. D Sequencing alignments of the DNA fragments with defined mutations in BMPR1B-edited and TBXT-edited animals