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. 2023 Jun 12;56:31. doi: 10.1186/s40659-023-00441-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — MEG3 binds to miR-21-3p, a direct target gene of miR-21-3p in PCOS rats. A Putative complementary binding site of miR-21-3p and MEG3; B Fluorophore activity assay verified the targeting relationship between MEG3 and miR-21-3p; C Experimental strategy for the inhibition of ovarian MEG3 expression by viral injection; D Detection of LncMEG3 by RT‒qPCR in ovarian granulosa cells of PCOS rats; E The expression of miR-21-3p was detected by RT‒qPCR in the ovarian granulosa cells of PCOS rats. A-E, N = 6. Data reflect all measurements and are expressed as the mean ± standard deviation. A t-test was used for statistical analysis between two groups, and one-way ANOVA and Tukey's post hoc test were used for statistical analysis between multiple groups. ***P < 0.0001 compared to the normal group; ^### P < 0.0001 compared to the sh-NC group