Fig. 4.

Silencing MEG3 increases the number of granulosa cells. A Experimental strategy for the inhibition of ovarian MEG3 expression by viral injection; B MEG3 downregulation of ovarian granulosa cells in PCOS rats; C Primary granulosa cells were extracted and cultured to observe cell number; The number of granulosa cells in PCOS rat ovaries were significantly increased after MEG3 downregulation (scale bar = 100 μm). A-C, N = 6. These data are expressed as the mean ± standard deviation, ***P < 0.0001 compared to the normal group; ^###P < 0.0001 compared to the sh-NC group and were statistically analysis using one-way ANOVA and Tukey's post hoc test. 1: Normal group, 2: PCOS group, 3:sh-MEG3 group, 4:sh-NC group