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. 2023 Jun 12;56:31. doi: 10.1186/s40659-023-00441-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Acupuncture intervention in PCOS rats increases the number of granulocytes. A Experimental strategy for acupuncture intervention; B Number of granulosa cells in the ovaries of PCOS rats after acupuncture intervention; C Primary granulosa cells were extracted and cultured to observe cell number. The number of granulosa cells in the ovaries of PCOS rats were significantly increased after acupuncture intervention (scale bar = 100 μm). A-C, N = 6.These data are expressed as the mean ± standard deviation, ***P < 0.0001 compared to the normal group and ^####P < 0.0001 compared to the sh-NC and PCOS groups. One-way factorial ANOVA and Tukey’s post hoc test were used for statistical analysis. 1: Normal group, 2: PCOS group, 3: PCOS + A group, 4: PCOS + SA group, 5: PCOS + W group