Table 1.
Gene name | Primer sequences (5’ to 3’) |
---|---|
LncMEG3 |
5′-GCTGGGTCGGCTGAAGAAC-3′ 5′-TGGCTGTGGAGGGATTTCG 3, |
miR-21-3p | 5′-AGCAGTCGATGGGCTGTCA-3′ |
Bcl-2 |
5′- CATGCGACCTCTGTTTGATTT-3′ 5′-TCACTTGTGGCCCAGGTATG-3′ |
bax |
5′-GATGAACTGGACAACAACATGGA-3′ 5′-CAAAGTAGAAAAGGGCAACCAC-3′ |
caspase-3 |
5′-GCAGCAGCCTCAAATTGTTGAC-3′ 5′-TGCTCCGGCTCAAACCATC-3′ |
β-actin |
5′-GGAGATTACTGCCCTGGCTCCTA-3′ 5′-GACTCATCGTACTCCTGCTTGCTG-3′ |
F forward, R reverse, LncMEG3 long noncoding RNA maternally expressed gene3, miR-21-3p microRNA-21-3p, Bcl-2 BCL2 mRNA, Bax BAX mRNA, caspase-3 Casp3 mRNA