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. 2023 Jun 7;10:20543581231165716. doi: 10.1177/20543581231165716

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© The Author(s) 2023

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Figure 1. — Shroom3 is expressed in the kidney tubular epithelium. (A and B) Immunofluorescence staining of adult 3-month-old wild type kidneys represent Shroom3 protein expression in the proximal convoluted tubules, distal convoluted tubules, and collecting ducts. Shroom3 expression levels is observed in the cortical tubules and medulla. (C) Immunofluorescence staining of adult 3-month-old wild type kidneys demonstrate no specific background staining of the anti-rabbit secondary antibody in the absence of the Shroom3 primary antibody. (D-L) Co-immunofluorescence staining of Shroom3 with known markers of the proximal convoluted tubules (SGLT2), distal convoluted tubules (SLC12A3), and collecting ducts (AQP2) confirm expression in these tubules. (J-L) Shroom3 is apically expressed in all the tubular epithelium analyzed. Scale bars = 50 µm (A-C). Scale bars = 25 µm (D-L).