Figure 2.

Heterozygous Shroom3 mutant kidney expression pattern. (A and B) Immunofluorescence staining in wild type and Shroom3^Gt/+mice demonstrates apical Shroom3 expression. Although reduced Shroom3 expression levels are observed in Shroom3^Gt/+mice expression remains apically localized. (C) Immunofluorescence staining of adult 3-month-old wild type kidneys demonstrates no specific background staining of the anti-rabbit secondary antibody in the absence of the Shroom3 primary antibody. (D-F) Total body weight at postnatal day 3, 1 month, and 3 months revealed no statistically significant differences indicating no abnormalities in somatic growth in Shroom3^Gt/+mice. (G-I) Kidney resection of wild type and Shroom3^Gt/+mice at postnatal day 3, 1 month, and 3 months. The kidney gross anatomy is similar in wild type and Shroom3^Gt/+mice. (J) Although, there were rare occurrences of Shroom3^Gt/+mice showing hypoplastic unilateral kidneys predominantly observed in the right kidney of heterozygous mice at 1 month. (H and I) Combined kidney weight analysis at postnatal 1 month and 3 months show no statistically significant differences. Scale bars = 10µm (A-B). Scale bars = 25µm (C).