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. 2023 Jun 7;10:20543581231165716. doi: 10.1177/20543581231165716

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© The Author(s) 2023

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Figure 4. — Shroom3^Gt/+mice exhibit modest alterations in apical-basolateral orientation in 3-month-old mice. (A-C) Immunofluorescence of postnatal day 3, 1 month, and 3 months wild type and Shroom3^Gt/+mice in the proximal convoluted tubules (SGLT2), distal convoluted tubules (SLC12A3), and collecting ducts (AQP2). (A) At postnatal day 3, SGLT2 and AQP2 show diffuse cytoplasmic expression. SLC12A3 shows strong apical localization in both wild type and Shroom3^Gt/+mice. (B) At postnatal 1 month, all markers show strong apical localization in both wild type and Shroom3^Gt/+mice. (C) At postnatal 3 months, all markers show strong apical localization in wild type mice. In Shroom3^Gt/+ mice, SGLT2 localization is more consistent with cytoplasmic staining while SLC12A3 and AQP2 expression pattern is similar between wild type and Shroom3^Gt/+mice. (D) Immunofluorescence staining of adult 3-month-old wild type kidneys demonstrate no specific background staining of the anti-rabbit secondary antibody in the absence of SLC12A3 as well as the anti-mouse secondary antibody in the absence of SGLT2 and AQP2. Scale bars = 25µm (A-D).