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. 2023 Jun 7;10:20543581231165716. doi: 10.1177/20543581231165716

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Shroom3^Gt/+mice do not exhibit proximal tubular and glomerular injury. (A) Immunohistochemistry for KIM-1 at postnatal day 3, 1 month and 3 months in wild type mice demonstrating no significant KIM-1 protein expression. (B) Immunohistochemistry for KIM-1 at postnatal day 3, 1 month and 3 months in Shroom3^Gt/+mice demonstrating no significant KIM-1 protein expression. (C) KIM-1 positive control for analysis. (D) Immunofluorescence staining of CD44 at postnatal 3 months in wild type and Shroom3^Gt/+ mice demonstrate no significant differences in expression levels. Shroom3^Gt/+ mice exhibit a thicker parietal epithelium than wild type mice. (E) Immunofluorescence staining of adult 3-month-old wild type kidneys demonstrate no specific background staining of the anti-mouse secondary antibody in the absence of the CD44 primary antibody. Scale bars = 50µm (A-E).