Table 3. Effect of silencing ESR1 on expression of AA metabolic pathway genes in MCF7 cells.
| GENE | Control | ESR1-Silenced | Fold Change | p-Value |
|---|---|---|---|---|
| FABP5 | 1566 ± 54 | 4101 ± 269 | 2.62 | 8.94e-05 |
| FABP7 | NE | NE | ||
| FADS1 | 298 ± 14 | 1597 ± 152 | 5.36 | 1.20e-04 |
| ACSL4 | 155 ± 10 | 1960 ± 75 | 12.6 | 1.99e-06 |
| PLIN2 | 95 ± 10 | 576 ± 87 | 6.06 | 3.40e-04 |
| PLA2G4A | 10 ± 0 | 128 ± 8 | 12.8 | 1.17e-05 |
| PTGS2 | 12 ± 2 | 81 ± 32 | 6.75 | 0.019 |
| ABCC4 | 211 ± 3 | 423 ± 36 | 2.00 | 5.60e-04 |
| PTGER4 | 201 ± 12 | 403 ÷ 103 | 2.00 | 0.022 |
| ALOX5 | 25 ± 1 | 25 ± 3 | 0 | 0.958 |
| ALOX12 | 29 ± 2 | 25 ± 1 | −1.16 | 0.047 |
| ALOX15 | 221 ± 3 | 27 ÷ 3 | −8.18 | 1.44e-07 |
| ESR1 | 4779 ± 66 | 21 ± 1 | −250 | 2.86e-08 |
| PGR | 382 ± 7 | 25 ± 3 | −15.4 | 1.49e-07 |
| AR | 903 ± 68 | 36 ± 1 | −25.6 | 2.51e-05 |
Data shown are mRNA expression values in arbitrary units. Abbreviations are as described for Figure 1 plus ALOX: arachidonate lipoxygenase; ESR1: estrogen receptor alpha; PGR: progesterone receptor; AR: androgen receptor; NE: not expressed. Calculations were made from array data reported by Al Saleh et al. [70]. Values shown represent the mean ± 1 SD of three separate data points.