Table 2.

Inter-day and intra-day analysis to determine the accuracy of the HPLC-UV method for levetiracetam estimation

Concentration (µg/ml)*	Calculated Concentration (mean±S.D.)	Accuracy (%)†
Intra-day (n=6)
5	5.14±0.03	109.43
20	22.26±0.43	110.6
40	43.83±0.08	109.41
Inter-day (n=18)
5	5.28±0.69	105.6
20	21.59±0.72	107.95
40	42.01±1.89	105.02

*Concentration of levetiracetam (μg/ml) obtained in the serum after spiking with the standard drug. ^†Accuracy of the method were assessed in percentage by executing replicate analysis of the 3 quality control samples. % Accuracy =(Accepted value – Observed value)/Accepted value*100