Appendix 2—figure 1. Experimental results and kinetic model for the transtion to 3D microcolonies.

(a) Comparison between the experimental data (markers) and the kinetic model (lines). Blue dots are data obtained with the pili-deficient mutant pilA: Tn5. Inset, residual of the fit of all the experimental data points as a function of the exponent value for $V_{CM}$, indicating that the best fit is achieved for a value of or close to 1. (b) ${\gamma }_{exp}$ values for all data points (black disks) and their average (red line). Gray squares are data points with ${\displaystyle v_0<2}$ μm.min^-1, showing a similar distribution and thus ruling out a significant bias at high velocities. (c) Same dataset as in a but the surface density at the transition is plotted; (d) same dataset as in a but the time at the transition is plotted. The same model is used to describe the data, but converted into the proper quantities.