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Extension of the primers PrI (Series A) and PrII (Series B) catalyzed by TDT. Series A: lane 1, primer + enzyme (control); lanes 2–5, as in lane 1 + 5 µM dATP (2), dGTP (3), dCTP (4) and dTTP (5); lane 6, as in lane 1 + 1 µM ddTTP (6). Series B: lane 1, primer + enzyme (control); lanes 2–9 as in lane 1 + 100 or 10 µM dATP (2 and 3), dGTP (4 and 5), dCTP (6 and 7), dTTP (8 and 9) and ddTTP (10 and 11), respectively.
