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Pyrophosphorolysis of the primers in complexes Tp1:PrI (Series A) and Tp1:PrII (Series B) catalyzed by DNA polymerase I. Lane 1, primer + template (control); lanes 2–4, as in lane 1 + enzyme for 1 (2), 3 (3) and 10 min (4), respectively; lanes 5–7, as in lane 1 + enzyme + 1 mM pyrophosphate for 1 (5), 3 (6) and 10 min (7), respectively.
