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(a) TLC of reaction products of complex Tp1:PrIV with dCTP and β-l-dTTP, when catalyzed by DNA polymerase I. Lane 1, complex Tp1:PrIV + enzyme; lanes 2–4, as 1 + dCTP, 5 (2), 50 (3) and 500 µM (4); lanes 5–7, as 1 + β-l-dTTP, 5 (5), 50 (6) and 500 µM (7); lanes 8 and 9 as 1 + PPi, 0.5 (8) and 1 mM (9). (b) Structure of l-dT-pppp-dA formed during pyrophosphorolysis of PrIV according to equation 1.
