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. 2023 Jun 8;8(11):e166698. doi: 10.1172/jci.insight.166698

Figure 3. RNF112 suppresses gastric cancer malignant behaviors in vivo.

Figure 3

(A) Tumors were harvested after s.c. injection of MGC803 cells with stable overexpression of RNF112 in nude mice. On day 19, tumors were removed from the sacrificed mice and used for subsequent analysis (n = 5). (B) Tumor growth curves of xenograft of the EV and RNF112 overexpression groups (n = 5). (C) Tumor weight was measured after the mice were sacrificed (n = 5). (D) Immunoblot analysis of FOXM1 and RNF112 expression of the tumors. (E) Quantitative reverse transcription–PCR analysis of FOXM1 downstream genes in the above tumors (n = 5). (F) IHC staining of PCNA, Ki67, FOXM1, and RNF112 in the indicated groups. (G) Bioluminescence imaging of representative mice and statistical analysis of the luminescence intensity (n = 5). (H) H&E staining of the representative metastatic lesions in the lung of nude mice and the statistical analysis of the relative tumor area (n = 5). Scale bar: 50 μm. Data are presented as mean ± SD. Statistical significance was calculated using Student’s t test (C, E, G, and H). *P < 0.05. Complete unedited blots are in the supplemental material.